Protein name Protein molecular weight (Da) Protein concentration (mg/mL) Volume of protein to be labeled (µL) Enter only one — either volume or amount of protein to be labeled Amount of protein to be labeled (mg) Molecular weight of labeling reagent (Da) Amount of labeling reagent used (mg) Maximum concentration of DMSO (%) * * Leave blank or 0 if not used Enter only one — either maximum concentration of DMSO or volume DMSO added to label Volume of anhydrous DMSO added (µL) Molar equivalents of labeling reagent used Volume of DMSO required to make a stock solution (µL) Volume of protein to be labeled (µL) Amount of stock labeling reagent to be added (µL) Amount of DMSO in labeling reaction (%)
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